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Michaelis–Menten constant (Km) and maximum velocity (vmax) values of recombinant N. benthamiana P4Hs using the synthetic peptide from IgA1 as substrate (VTVPVPSTPPTPSPSTPPTPSPS).
| Recombinant enzyme | Km (μM) | vmax (μM/min) |
| Nb-P4H1 | 8 | 0.03 |
| Nb-P4H4 | 1900 | 0.1 |
| Nb-P4H9 | 72 | 0.4 |
| Nb-P4H10 | 66 | 0.02 |
Km and vmax values were calculated based on Hanes-Woolf plots Supplementary Figure 4, panel B1-B4.
