Table 1.
Primer pairs used to amplify cDNA.
| Gene | Direction/position of primer | Sequence |
|---|---|---|
| 16S rRNAa | Forward, 469-491 | 5′-TTGACGGTAGGTGGAGAAGAAGC-3′ |
| Reverse, 909-888 | 5′-CCTTTGAGTTTTAGCCTTGCGG-3′ | |
| narJ | Forward, 439-512 | 5′-CCTATGAGTACACCGTGGCG-3′ |
| Reverse, 603-584 | 5′-GGGACGGTCAAGGTAAACGG-3′ | |
| tcrY | Forward, 796-815 | 5′-CATGAACTGCGAACTCCCCT-3′ |
| Reverse, 930-911 | 5′-GACGAGACGTGTTATCCGCT-3′ | |
| uvrC | Forward, 114-133 | 5′-CGAGTCATCTACGTCGGCAA-3′ |
| Reverse, 308-289 | 5′-GAATCGCGGATCGAACTCCT-3′ | |
| Rv1356c | Forward, 531-550 | 5′-CCTACCGCTAAGCATGTCCC-3′ |
| Reverse, 643-624 | 5′-CCGGGATTCTCGCTGCTATT-3′ | |
| Rv3134c | Forward, 472-491 | 5′-GAGGTGGACAATGGTGTGGT-3′ |
| Reverse, 610-591 | 5′-TTCGACGTCATCGGGTGTTT-3′ |
a
The 16S rRNA gene was used as a housekeeping gene to normalize the data. Primers from Haile et al. (2002) [13].