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. 2021 Mar 2;11:610547. doi: 10.3389/fonc.2021.610547

Figure 2.

Figure 2

Upregulation of TGM2 expression promoted the migration and invasion of PTC cells. (A) Representative photographs from IHC analysis of TGM2 protein levels in normal and tumor samples, with or without lymph node metastasis and extrathyroidal extension. Scale bars: 50 μm. (B) Western blotting analysis of relative TGM2 protein levels in 12 pairs of PTC tissues (T) and corresponding adjacent non-cancerous tissues (N). *P < 0.05, **P < 0.01 versus adjacent non-cancerous tissues. (C) The relative mRNA expression of TGM2 was detected using qRT-PCR in PTC cells after transfection with si-TGM2 or NC. Data are presented as the mean ± S.D., as analyzed using an independent samples t-test. **P < 0.01 versus NC. (D) Western blotting was applied to detect the protein level of TGM2 in PTC cells transfected with si-TGM2 or NC. Data are presented as the mean ± S.D., as analyzed using an independent samples t-test. **P < 0.01 versus NC. (E) Transwell assays were used to evaluate the migration and invasion in PTC cells after transfection with si-TGM2 or NC. Data are presented as the mean ± S.D., as analyzed using an samples t-test. **P < 0.01 versus NC. (F) A wound healing assay was applied to analyze the migration capacity in PTC cells after transfection with si-TGM2 or NC. All data are presented as the mean ± S.D.