Figure 3.

Overexpression of TGM2 partially impairs NEAT1_2 depletion-mediated migration and invasion in PTC cells or in vitro. (A) Western blotting was used to analyze the level of TGM2 in PTC cells transfected with si-NEAT1_2 or co-transfected pCDNA3.1-TGM2 and si-NEAT1_2 or NC. Data are presented as the mean ± S.D., as analyzed using an independent samples t-test. **P < 0.01 versus NC. (B) Transwell assay analysis was used to evaluate the migration and invasion of PTC cells after transfection with si-TGM2 or after co-transfection pCDNA3.1-TGM2 and si-NEAT1_2 or NC. Data are presented as the mean ± S.D., as analyzed using an independent samples t-test. **P < 0.01 versus the si-NEAT1_2 group. (C) A wound healing assay was applied to analyze the migratory capacity of PTC cells after transfection with si-NEAT1_2 or co-transfected pCDNA3.1-TGM2 and si-NEAT1_2 or NC. (D) Lung metastasis nodules from model mice after intravenous injection of 1 × 10⁶ K1 cells of the LV-sh-NEAT1_2 group, the LV-empty vector group, and the LV-shRNA-NEAT1_2 + pcDNA-TGM2 group. Data are presented as the mean ± S.D., as analyzed using an independent samples t-test. **P < 0.01 versus LV-empty vector group, ^##P < 0.01 versus LV-sh-NEAT1_2 group. (E) IHC staining to analyze FN1, NFκb, and TGM2 expression in the mouse lung tissue.