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. 2021 Mar 2;11:620907. doi: 10.3389/fonc.2021.620907

Figure 7.

Figure 7

The role of AKT/GSK3β/β-catenin signaling in Hsp90-induced multi-drug resistance (MDR). Respective western blots and relative quantitation for AKT, p-AKT, p-GSK3β, GSK3β, total β-catenin, non-phospho (active) β-catenin, and Hsp90 in A2780 cells transfected with pcDNA3.1, pcDNA3.1-Hsp90, or shAKT plus pcDNA3.1-Hsp90 for 72 h (A,B), A2780/Taxol cells transfected with shNC, shHsp90, or pcDNA3.1-Hsp90 plus shHsp90 for 72 h, and then treated with or without MK2206 for 6 h (C,D), A2780/Taxol cells incubated with DMSO, BIIB021, or BIIB021 plus transfection with pcDNA3.0-AKT for 72 h (E,F), and A2780/Taxol cells transfected with pcDNA3.0, pcDNA3.0-D88N-Hsp90, or pcDNA3.0-D88N-Hsp90 plus pcDNA3.0-AKT for 72 h (G,H). Dual-luciferase reporter assay for TOPflash and FOPflash luciferase activity in A2780 cells transfected with pcDNA3.1, pcDNA3.1-Hsp90, or shAKT plus pcDNA3.1-Hsp90 for 48 h (I), A2780/Taxol cells transfected with shNC, shHsp90, or pcDNA3.1-Hsp90 plus shHsp90 for 48 h, and then treated with or without MK2206 for 6 h (J), A2780/Taxol cells incubated with DMSO, BIIB021, or BIIB021 plus transfection with pcDNA3.0-AKT for 48 h (K), and A2780/Taxol cells transfected with pcDNA3.0, pcDNA3.0-D88N-Hsp90, or pcDNA3.0-D88N-Hsp90 plus pcDNA3.0-AKT for 48 h (L). The sensitivity and IC50 of paclitaxel (M,N) or cisplatin (O,P) in A2780 cells transfected with pcDNA3.1, pcDNA3.1-Hsp90, or pcDNA3.1-Hsp90 plus shAKT for 72 h. The sensitivity and IC50 of paclitaxel (Q,R) or cisplatin (S,T) in A2780/Taxol cells transfected with shNC, shHsp90, or shHsp90 plus pcDNA3.0-AKT for 72 h. The results of western blotting were analyzed using ImageJ (mean ± SD of 3 independent experiments). *P < 0.05.