Table 1.
Techniques for detection and/or quantification of lipopeptide production.
| Technique | Advantages | Disadvantages |
|---|---|---|
| Blood agar lysis | Ease of use | Not specific and not reliable |
| Drop collapse | Ease of use | Not specific |
| Oil spreading | Ease of use, better prediction than drop collapse | Not specific |
| Surface tension measurement | Ease of use, reliable | Not specific |
| Color shift | Ease of use, high-throughput | Not specific |
| HPLC-UV | Can discriminates the different lipopeptides if standard, quantification possible | Expensive equipment |
| LC-MS | Discriminates the different lipopeptides | Expensive equipment |
| PCR or genome sequencing | Production capacity measurement | Observes only genes |
| RT-PCR | Production capacity measurement | Observes only gene transcription |