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. 2021 Mar 2;15:641970. doi: 10.3389/fnins.2021.641970

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Copyright © 2021 Namestnikova, Gubskiy, Revkova, Sukhinich, Melnikov, Gabashvili, Cherkashova, Vishnevskiy, Kurilo, Burunova, Semkina, Abakumov, Gubsky, Chekhonin, Ahlfors, Baklaushev and Yarygin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phenotypic characterization and differentiation of pMSC. Chondrogenic (A), osteogenic (B) and adipogenic (C) differentiation of pMSC, revealed by Alcian Blue, Alizarin Red and Oil Red staining, respectively. Scale bar = 100 μm. Flow cytometry analysis of the pMSCs’ surface antigen expression (D). Most cultured cells were positive for surface antigens typically expressed by mesenchymal stem cells: CD105+, CD29+, CD73+, CD90+ and negative for non-mesenchymal CD34- and CD45- markers. Blue graphs represent negative control (isotypic immunoglobulins); pink graphs – experimental samples.