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. 2021 Mar 2;15:641970. doi: 10.3389/fnins.2021.641970

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Copyright © 2021 Namestnikova, Gubskiy, Revkova, Sukhinich, Melnikov, Gabashvili, Cherkashova, Vishnevskiy, Kurilo, Burunova, Semkina, Abakumov, Gubsky, Chekhonin, Ahlfors, Baklaushev and Yarygin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Location of drNPC inside the cerebral vessels after IA infusion. Top panel represents the SWI of the rat brain before and 30 min and 24 h after transplantation. White rectangle indicates the area of SPIO-labeled cell accumulation. Bottom panel demonstrates high-magnification confocal fluorescence microscopy images of double-labeled drNPC (PKH26 – orange, SPIO microparticles in the cytoplasm – green) inside the cerebral blood vessels (stained with EBA, red). Scale bars: 20 μm. Bottom right insert: 3D-reconstruction of z-stacks demonstrated the cells’ localization inside vessels.