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. 2021 Mar 15;11:5953. doi: 10.1038/s41598-021-85306-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of Dicer and AGO2 knockdown. (a) Relative expression of Dicer mRNA was significantly downregulated (*P < 0.01) in DicerKD compared to WT hMSCs after 1, 7 and 14 days cultured in osteogenic medium. (b) Relative expression of Argonaute2 mRNA was significantly downregulated (*P < 0.01) in AGO2KD compared to WT hMSCs after 1, 7 and 14 days cultured in osteogenic medium. Fold changes are calculated relative to WT day 1 and Mann–Whitney U test was utilized for statistical analyses. (c) Immunoblotting showed successful knockdown of Dicer and Argonaute2 proteins in DicerKD and AGO2KD cells, respectively. Regions of interest of scans of one nitrocellulose blot probed with either anti-Dicer or anti-AGO2 or anti-tubulin primary antibody and corresponding secondary antibodies are shown.