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Schematic of experimental design for exosomal complementation study: WT hMSC cultures were initiated 24 h prior to DicerKD and AGO2KD cultures. At designated times, WT hMSCs were grown 24 h in serum free media before collection of media for isolation of WT hMSC exosomes. The following day, WT hMSC exosomes were added to DicerKD and AGO2KD cultures. The cells were harvested at day 1 and after 7 or 14 days for analysis of osteoblastic gene expression and culture mineralization.
