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. 2021 Mar 15;11:5953. doi: 10.1038/s41598-021-85306-2

Table 3.

Primer sequences used for RT-qPCR.

Gene Forward (5′ → 3′) Reverse (5′ → 3′) Amplicon size (bb)
GAPDH CAGGGCTGCTTTTAACTCTGG TGGGTGGAATCATATTGGAACA 102
DICER GAGCTGTCCTATCAGATCAGGG ACTTGTTGAGCAACCTGGTTT 92
ARGOUNATE 2 TCCACCTAGACCCGACTTTGG GTGTTCCACGATTTCCCTGTT 157
RUNX2 TGGTTACTGTCATGGCGGGTA TCTCAGATCGTTGAACCTTGCTA 101
OSX (Sp7) CCTCTGCGGGACTCAACAAC AGCCCATTAGTGCTTGTAAAGG 122
BSP (SPP1) GAAGTTTCGCAGACCTGACAT GTATGCACCATTCAACTCCTCG 91
Collagen I GAGGGCCAAGACGAAGACATC CAGATCACGTCATCGCACAAC 140