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. 2021 Mar 15;11:5916. doi: 10.1038/s41598-021-84265-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Molecular phylogeny of the Chlorellaceae based on SSU and ITS sequence comparisons. The phylogenetic trees shown were inferred using the maximum likelihood method based on the data sets (2604 aligned positions of 31 taxa) using PAUP 4.0a166. For the analyses, the best model was calculated by Modeltest 3.7. The setting of the best model was given as follows: GTR+I+G (base frequencies: A 0.2160, C 0.2732, G 0.2728, T 0.2380; rate matrix A–C 0.8499, A–G 1.2840, A–U 1.1061, C–G 0.6217, C–U 3.1850, G–U 1.0000) with the proportion of invariable sites (I = 0.6996) and gamma shape parameter (G = 0.5575). The branches in bold are highly supported in all analyses (Bayesian values > 0.95 calculated with MrBayes, 5 million generations; bootstrap values > 70%, calculated with PAUP, 1000 replicates using maximum likelihood, neighbour-joining, and maximum parsimony). The endosymbionts that were previously detected in Paramecium bursaria are marked with an asterisk.