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. 2021 Mar 15;11:5877. doi: 10.1038/s41598-021-85230-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Differentiation of PE- and control-iPSC into CTB-like cells. (A) Upper panel: Representative flow cytometric analysis of CTB marker, EGFR, as compared to isotype control, following differentiation of iPSC into CTB-like cells (after 4 days of BMP4 + IWP2 treatment). Lower panel: Bar chart displaying average percent EGFR positive cells from both PE- and control-iPSC at day 4 of differentiation, ± standard deviation (n = 9 for each condition). (B) Box plot displaying qPCR of CTB markers p63 and CDX2 at day 4 of differentiation, normalized to L19, and expressed as fold change over undifferentiated control-iPSC (day 0) (n = 9 for each condition).