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. 2021 Mar 15;11:5877. doi: 10.1038/s41598-021-85230-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Response of iPSC-derived trophoblast to changes in oxygen tension. (A) Invasive capacity was assessed by Matrigel invasion assay and (B) hCG secretion was measured by hCG ELISA and normalized to its DNA content. Only control iPSC-derived trophoblast (but not PE iPSC-derived trophoblast) show a statistically significant response to a lowering of oxygen tension. *Indicates statistical significance by Wilcoxon Signed Rank Test, with p values as stated (n = 9 for each condition).