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. 2021 Mar 15;12(3):280. doi: 10.1038/s41419-021-03562-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A–D Percentages of T_h17 cells and Tregs in PDX-treated Nlrp3^+/+ and Nlrp3^−/− CIA mice as shown by FCM (respectively, F[6,18] = 61.58 and F[6,18] = 31.29). E Ratio of Tregs to T_h17 cells in PDX-treated Nlrp3^+/+ and Nlrp3^−/− CIA mice (F[6,18] = 31.70). F mRNA expression of RORγ and FOXP3, and FOXP3/RORγ ratio, in lymphocytes from PDX-treated Nlrp3^+/+ and Nlrp3^−/− CIA mouse lymph nodes as shown by RT-qPCR (F[6,18] = 13.62 for RORγ, F[6,18] = 9.584 for FOXP3 and F[6,18] = 37.08 for FOXP3/RORγ). Data were presented as means ± SD. Differences among the three groups were assessed by one-way ANOVA, and post hoc tests (Tukey’s method) were applied to investigate the differences one by one.