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. 2021 Mar 15;11:162. doi: 10.1038/s41398-021-01280-3

Fig. 1. Ngf-knockout mice show decreased hippocampal neurogenesis and depleted cholinergic innervations.

Fig. 1

A Ngf floxed mice were crossed with Sox1::Cre mice to knock out Ngf specifically in the brain from early development. B We confirmed the high knockout efficiency of Ngf in the hippocampus at mRNA (n = 6). C Images of neurogenesis markers DCX, Ki67, and BrdU and cell counts in the dentate gyrus of the hippocampus. There were significantly decreased cell counts of Ki67- and DCX-positive cells in the Ngf cKO hippocampus (n = 8–10). For the BrdU/NeuN-positive cell counts, Ngf cKO mice also exhibited decreased cell counts (n = 7–9). D Images and quantification of ChAT in the hippocampus. Ngf cKO mice showed depleted cholinergic fiber in the hippocampus (n = 5). E Representative images of MRI scans and quantification of hippocampal volume (n = 7). Ngf cKO hippocampi are smaller in volume compared to WT. Decreased hippocampal weight in Ngf cKO mice were consistent with the result from the MRI (n = 12–13). Data shown as mean wet weight of bilateral hippocampus.