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. 2021 Mar 15;11:162. doi: 10.1038/s41398-021-01280-3

Fig. 3. Overexpression of hippocampal Ngf in knockout mice restore cholinergic innervations and spatial memory.

Fig. 3

A, B Hippocampal Ngf overexpression (using AAV-Ngf) on the Ngf cKO background had no effect on the open field or elevated plus maze (n = 19–20). C, D Ngf overexpression did not improve spatial learning (n = 8–9), but did improve spatial memory retention. E Images of Ngf overexpression in the hippocampus by in situ hybridization. F We confirm the Ngf overexpression in the hippocampus by real-time PCR (n = 5–6). G, H Images of cholinergic fibers in AAV-eGFP control and AAV-Ngf mice by ChAT staining. Quantification of fiber density revealed that Ngf overexpression partially restored cholinergic fibers in the hippocampus (n = 6). Dotted lines represent the levels of age-matched WT naive mice.