Fig. 4. Hippocampal cholinergic depletion causes impaired spatial memory but no effect on anxiety.

A, B C57 Mice injected in the hippocampus with anti-p75^NTRSaporin to deplete cholinergic fibers (hippocampal cholinergic depletion) were not different to controls on the open field or elevated plus maze (n = 7–10). C, D Hippocampal cholinergic depletion did not effect spatial learning but impaired spatial memory. E Images of ChAT staining in PBS or anti-p75^NTRSaporin-injected hippocampus. We confirmed that mice with anti-p75^NTRSaporin injection had a profound decrease in cholinergic fiber density in the hippocampus without affecting the cortex (n = 6–9).