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. 2021 Mar 15;11:162. doi: 10.1038/s41398-021-01280-3

Fig. 5. Overexpression of hippocampal Ngf in knockout mice with cholinergic depletion cannot rescue spatial memory.

Fig. 5

A, B Hippocampal Ngf overexpression (AAV-Ngf) in WT mice that had hippocampal cholinergic depletion using anti-p75NTRSaporin had no effect on the open field or elevated plus maze compared to hippocampal cholinergic-depleted mice given AAV-eGFP injection as control (n = 15–20). C, D Ngf overexpression in hippocampal cholinergic-depleted mice did not affect spatial leaning or memory (n = 19–21). E, F ChAT staining confirmed cholinergic depletion in both groups. G We confirmed the Ngf overexpression by real-time PCR (n = 6–8). Dotted lines represent the levels of age-matched WT naive mice.