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. 2021 Mar 15;11:6055. doi: 10.1038/s41598-021-84888-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Osteoblast (OBL) and osteoclast (OCL) numbers temporally fluctuate dynamically as bone heals from injury. (a) Schematic summarizing published dynamics of OBL and OCL following bone injury. (b) Schematic depicting the experimental workflow to induce bone injury in mice and generate bone, osteoblast and osteoclast dynamic data. (c) Representative images of micro-computed tomography revealed trabecular bone status (BONE). Decalcified bones were stained and quantified for OBL by RUNX2 immunofluorescence staining (OBL), and OCL by tartrate-resistant acid phosphatase (TRAcP) staining (OCL). (d) Quantitation of temporal dynamics of bone volume, osteoclast and osteoblast population.