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. 2021 Mar 15;11:5932. doi: 10.1038/s41598-021-85455-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Lipid profiling in WAT and BAT of young and aging mice. (A) Experimental design: 2 and 24 months old mice were sacrificed every 4 h; WAT and BAT was harvested and lipids were extracted; lipidomics and data analysis was performed; and the obtained lipid profiles were analyzed with JTK_CYCLE analysis for detection of oscillating lipids. (B) The abbreviations and common names of the 19 detected lipid classes. The lipid composition of young mice in WAT (C), BAT (D), and of aged mice in WAT (E) and in BAT (F). Illustration and figure layout were prepared in Adobe Illustrator CS6 (v16.0.3; https://www.adobe.com/nl/). Pie charts were created in Graphpad Prism (v8.3.0; https://www.graphpad.com/scientific-software/prism/).