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. 2021 Mar 15;11:5932. doi: 10.1038/s41598-021-85455-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Aged BAT accumulates DAG and BMP. (A) Principal component analysis of young and aged mice BAT lipidome shows clear separation. (B) Heatmaps of all lipid classes arranged from left to right by lipid abundance and ordered from top to bottom by acyl chain length. Relative abundance of (C) sterol and glycerolipids; (D), (E) glycerophospholipids and sphingomyelins show accumulation of BMPs and DAGs. **P < 0.01, ***P < 0.001 (unpaired Student’s t-test) (n = 4). PCA analysis and heatmaps were created in R programming language (v3.6.3; http://www.r-project.org). Bar graphs were created in Graphpad Prism (v8.3.0; https://www.graphpad.com/scientific-software/prism/). Figure layout was prepared in Adobe Illustrator CS6 (v16.0.3; https://www.adobe.com/nl/).