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. 2021 Mar 15;11:5932. doi: 10.1038/s41598-021-85455-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Rhythmic lipid profiles is influenced by acyl chain length in young BAT. (A) Percentage of rhythmic lipids in young and (C) aged mice as considered by JTK_CYCLE analysis. The rhythmic lipids contained lipids of different lipid classes. Depiction of the rhythmic/lipid class percentage of young (B) and (D) aged mice (n = 4 per time point). Double plotted rhythmic profiles of (E) DAGs, (F) TAGs and (G) PCs in young mice compared to aged mice. The gray lines represent the individual lipid species and the bold colored lines the group average. The peak time point of individual lipids species depicted with the color to their corresponding lipid class of young (H) and (I) aged mice. Graphs were created in Graphpad Prism (v8.3.0; https://www.graphpad.com/scientific-software/prism/). Figure layout was prepared in Adobe Illustrator CS6 (v16.0.3; https://www.adobe.com/nl/).