Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Three developed intra-genic markers used to amplify Cd accumulating genes.
| Gene | Marker | Primer sequence | Expected size (bp) of IRAT129 and 9311 (IRAT129/9311) | Restriction enzyme | |
|---|---|---|---|---|---|
| Forward (5′-3′) | Reverse (5′-3′) | ||||
| OsHMA3 | HMA3 | AATCCATCCAACCAAACCCGGAA | ATGCCCAGCGATCCAAGCTC | 280 bp | BsaHI |
| OsNramp5 | Nramp5 | TTGTCATGCCGTACGCTT | CACCAAGGCAGAATGCAA | 140/144 bp | |
| OsNramp1 | Nramp1 | GGCTGTAGATACCACATCACC | GCTGCCTCAAGAATTATACCTG | 761/355 bp | |
