Figure 3.

Microscopy analysis and detection of glucose modifications on WTA produced by aerobically grownWTBacillus subtilis and mutant and complementation strains. The indicated B. subtilis strains were grown under aerobic growth conditions to the mid-log phase in LB medium supplemented with IPTG and glucose. Bacteria were prepared for microscopy analysis and stained with the fluorescently labeled Alexa Fluor 594 concanavalin A lectin to detect glucose modifications on WTA as described in the Experimental procedures section. The experiment was performed 3 times and representative phase-contrast, fluorescence, and merged images are shown for each strain. Scale bars represent 10 μm. WTA, wall teichoic acid.