Skip to main content
. 2021 Feb 7;296:100391. doi: 10.1016/j.jbc.2021.100391

Figure 3.

Figure 3

The CuRE sequences are required for proper regulation of SOD1 during Cu limitation. A, schematic representation of consensus CuRE sequences (blue boxes) and number (red text) within the promoters of SOD1 and SOD2. Solid green boxes represent exons and connecting lines represent introns. The yellow box depicts the amino-terminally encoded mitochondrial import peptide (MIP) of SOD2. Numbers below CuREs represent start of the sequences relative to the initiating codon (+1). B, consensus motif of the CuRE sequences identified using the MEME suite software (46). C, qRT-PCR analysis of SOD1 transcript levels in the indicated strains when cultured with 1 mM BCS (gray) or 0.1 mM CuSO4 (blue). Expression levels were normalized to the SOD1C strain. N = 3, 2-tailed t test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.005. D, protein expression levels of Sod1 in the indicated strains treated with 1 mM BCS (B) or 0.1 mM CuSO4 (C) for 12 h. Sod1 was detected using the yeast anti-Sod1 antibody (ySod1) as well as an anti-FLAG epitope antibody (FLAG). Detection of Cdc2 was included as a loading control. CuRE, copper responsive element; SOD, superoxide dismutase.