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. 2021 Feb 7;296:100391. doi: 10.1016/j.jbc.2021.100391

Figure 4.

Figure 4

Cuf1 regulates SOD2 expression through CuRE sequences resulting in an alternative transcript shortened at the 5’-end. A, ChIP-qPCR of the SOD2 promoter region in the SOD2C and SOD2-CuRE1/2mut strains containing the CUF1-FLAG allele when treated with 1 mM BCS. N = 3, 2-tailed t test. B, qRT-PCR analysis of SOD2 transcript levels in the SOD2C and SOD2-CuRE1/2mut strains when treated with 1 mM BCS or 0.3 mM CuSO4. N = 3, 2-tailed t test. C, visualization of final 5’-RACE PCR products of SOD1 and SOD2 from WT C. neoformans cells treated with 1 mM BCS or 5 μM CuSO4. Products were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. D, Same as in (C), but using the cuf1Δ strain and the SOD2-CuRE1/2mut strain. ∗p < 0.05, ∗∗p < 0.01, n.s., not significant. BCS, bathocuproinedisulfonic acid; CuRE, copper responsive element; FL, full length; TR, truncated, shortened; SOD, superoxide dismutase.