Figure 9.

Cytosolic Sod2 is required for colonization of Cu-deficient host niches using murine models of fungal infection. A, retro-orbital administration of 500 cells of the indicated strains resuspended in 1X PBS into A/J male and female mice. Shown are mouse survival plots with percent survival plotted as a function of days postinfection. Strain genotypes are indicated, and mock infected mice were administered an equal volume of 1X PBS. The survival average of mice infected with the wildtype (WT) was compared with the survival average of mice infected with any of the mutants by the log rank statistical test. N = 10. B, C. neoformans CFU analysis of mouse organs 3 days postinfection. Shown are the indicated C. neoformans genotypes. Statistical differences were assessed across groups by the nonparametric Kruskal-Wallis one-way analysis of variance. F-test for variances was used between sample groups and significance determined by a 2-tailed t-test. N = 3. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.005. CFU, colony forming unit.