Table 1.
Bone phenotype of third and fourth vertebral body from 14-week-old female sham versus ovariectomized (OVX) Dkk1fl/fl;Rosa26-CreERT2, Dkk1fl/fl;Osx-Cre and Dkk1fl/fl;Dmp1-Cre mice
| Spine | Cre− Sham | OVX | p value and % change | Cre+ Sham | OVX | p value and % change |
|---|---|---|---|---|---|---|
| Dkk1fl/fl;Rosa26-CreERT2 | n = 12 | n = 12 | n = 10 | n = 12 | ||
| μCT | ||||||
| Tb.N [1/mm] | 3.87 ± 0.35 | 3.61 ± 0.19 | 0.225–7% | 4.38 ± 0.32## | 4.17 ± 0.34### | 0.682–5% |
| Tb.Sp [mm] | 0.25 ± 0.03 | 0.27 ± 0.02 | 0.647 + 8% | 0.24 ± 0.04 | 0.26 ± 0.02 | 0.567 + 8% |
| Histomorphometry | ||||||
| MS/BS [%] | 17.7 ± 4.02 | 20.0 ± 2.75 | 0.787 + 13% | 18.6 ± 1.91 | 20.1 ± 4.51 | >0.999 + 8% |
| MAR [μm/day] | 1.26 ± 0.15 | 1.61 ± 0.25 | <0.05 + 28% | 1.69 ± 0.25## | 1.92 ± 0.34 | 0.351 + 13% |
| N.Ob/B.Pm [#/mm] | 11.9 ± 3.45 | 12.4 ± 3.39 | >0.999 + 4% | 13.1 ± 2.80 | 14.2 ± 4.05 | >0.999 + 8% |
| N.Ot/B.Ar [#/mm2] | 152 ± 49.2 | 184 ± 28.8 | 0.228 + 17% | 252 ± 34.4### | 244 ± 19.8## | >0.999–3% |
| Dkk1fl/fl;Osx-Cre | n = 16 | n = 16 | n = 12 | n = 13 | ||
| μCT | ||||||
| Tb.Th [mm] | 40.4 ± 2.28 | 36.7 ± 2.42 | <0.01–9% | 41.4 ± 3.01 | 38.2 ± 2.31 | <0.05–8% |
| Tb.N [1/mm] | 3.56 ± 0.41 | 3.22 ± 0.27 | <0.05–10% | 4.02 ± 0.24## | 3.67 ± 0.23## | <0.05–9% |
| Tb.Sp [mm] | 0.28 ± 0.03 | 0.31 ± 0.02 | <0.05 + 11% | 0.25 ± 0.02## | 0.27 ± 0.02## | <0.05 + 8% |
| Histomorphometry | ||||||
| MS/BS [%] | 25.2 ± 5.31 | 29.1 ± 4.60 | 0.246 + 15% | 28.2 ± 4.21 | 28.6 ± 3.72 | +1% |
| MAR [μm/day] | 1.49 ± 0.45 | 2.29 ± 0.55 | <0.05 + 54% | 2.44 ± 0.91## | 3.30 ± 0.90## | <0.05 + 35% |
| BFR/BS [μm3/μm2/day] | 0.40 ± 0.16 | 0.75 ± 0.33 | <0.05 + 88% | 0.78 ± 0.38# | 1.15 ± 0.30## | <0.05 + 47% |
| N.Ob/B.Pm [#/mm] | 16.2 ± 3.03 | 17.2 ± 2.56 | >0.999 + 6% | 16.4 ± 2.72 | 16.5 ± 3.54 | >0.999 + 1% |
| N.Oc/B.Pm [#/mm] | 7.06 ± 1.84 | 8.91 ± 1.77 | <0.05 + 26% | 4.39 ± 1.09### | 6.24 ± 1.67### | <0.05 + 42% |
| Dkk1fl/fl;Dmp1-Cre | n = 13 | n = 15 | n = 13 | n = 10 | ||
| μCT | ||||||
| Tb.Th [mm] | 41.0 ± 3.64 | 38.4 ± 2.85 | 0.087–6% | 42.7 ± 2.24 | 39.6 ± 1.70 | 0.066–7% |
| Tb.N [1/mm] | 3.70 ± 0.33 | 3.29 ± 0.32 | <0.05–11% | 4.10 ± 0.32# | 3.71 ± 0.33# | <0.05–10% |
| Tb.Sp [mm] | 0.28 ± 0.02 | 0.31 ± 0.03 | <0.01 + 11% | 0.25 ± 0.02# | 0.28 ± 0.03# | <0.05 + 12% |
| Histomorphometry | ||||||
| MS/BS [%] | 28.9 ± 3.60 | 30.1 ± 2.60 | >0.999 + 4% | 28.9 ± 3.57 | 29.9 ± 2.62 | >0.999 + 3% |
| MAR [μm/day] | 1.39 ± 0.23 | 1.95 ± 0.44 | <0.01 + 40% | 1.96 ± 0.36## | 2.42 ± 0.34# | <0.05 + 23% |
| BFR/BS [μm3/μm2/day] | 0.37 ± 0.17 | 0.62 ± 0.16 | <0.05 + 68% | 0.70 ± 0.24## | 1.03 ± 0.22### | <0.01 + 47% |
| N.Ob/B.Pm [#/mm] | 23.2 ± 3.36 | 23.7 ± 3.06 | >0.999 + 2% | 23.6 ± 4.51 | 24.6 ± 3.58 | >0.999 + 4% |
| N.Oc/B.Pm [#/mm] | 8.02 ± 2.00 | 11.2 ± 2.58 | <0.001 + 40% | 5.90 ± 1.28# | 8.47 ± 1.30## | <0.05 + 44% |
Tb.Th = trabecular thickness, Tb.N = trabecular number, Tb.Sp = trabecular separation, MS/BS = mineralizing surface/bone surface, MAR = mineral apposition rate, BFR/BS = bone formation rate/bone surface, N.Ob/B.Pm = number of osteoblasts, N.Oc/B.Pm = number of osteoclasts/bone perimeter, N.Ot/B.Ar = number of osteocytes/bone area. Data represent the mean ± SD. Statistical analysis was performed using the two-way ANOVA. #p < 0.05, ##p < 0.01, ###p < 0.001 versus respective Cre-negative control.