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. 2021 Mar 15;11:5950. doi: 10.1038/s41598-021-85276-5

Figure 4.

Figure 4

Developing murine dermal fibroblasts have emergent morphology-mechanosensing relationships. Murine dermal fibroblasts from adults and embryonic days 15 and 18 were isolated and seeded onto organized and disorganized fiber environments. (a) Staining for actin, the nucleus, and YAP/TAZ revealed heterogeneity and developmental stage specific morphology and mechanosensing. (b) The neural network described above (Fig. 3) was updated and retrained based on AFCs, MSCs, and adult murine dermal fibroblasts (mDFs) and tested on the remaining dermal fibroblast groups (c). In general, YAP/TAZ levels were predictable for (d) adult and (e) E18 cells (µadult = − 0.24, σadult = 0.29; µe18 = − 0.21, σe18 = 0.90). (f) However, YAP/TAZ levels were largely overestimated for dermal fibroblasts isolated from the mid-gestational fetus (E15), which is a stage associated with scarless healing (µe15 = − 1.17, σe15 = 1.32), (nDF-train = 65, nDF-test = 18, nE18 = 95, and nE15 = 47 cells).