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. 2021 Mar 15;12(3):279. doi: 10.1038/s41419-021-03541-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — A The ideograph of ORF-CISD1 lentivirus injection site. B The protein expression of CISD1 increased after injection of ORF-CISD1 lentivirus. C TTC staining of rat brains. Scale bar = 1 cm. D Infarcts were quantified by planimetry and expressed as a percentage of risk zones. E Zea-longa score of HI rats. F Grip times were assessed in 1 d, 3 d, and 7 d after HI. G Time on rotarod was assessed in 38 d after HI. H Number of crossing was recorded in 38 d after HI. I Latency to platforms were assessed in 30 d, 31 d, 32 d, 33 d, and 34 d after HI. J, K Reduction of right cerebral blood flow after HI. L Representative EM images of autophagic vacuoles were shown. Scale bar = 250 nm. The arrows depict autophagosomes. M–Q The expression of ATG12, P62, Beclin-1 LC3I, and LC3II were detected by western blotting. Representative immunoblot for conversion of LC3I to LC3II in Sham group, HI group, ORF-NC group and ORF-CISD1 group. The densitometric analysis of WB of LC3II is shown. Data are shown as mean ± s.d. *P < 0.05, **P < 0.01 with one-way ANOVA, n = 6. CISD1 CDGSH iron-sulfur domain-containing protein 1, HI hypoxia ischemia, ORF-NC negative control overexpression, ORFCISD1 CISD1 overexpression, H hours, S seconds.