Figure 5.

Tolerance induced by FR70 resulted in the acceptance of secondary donor-type cardiac grafts after adoptive splenocyte transfer. The splenocytes harvested at POD60 and 100 from the C3H recipients bearing B6 grafts were adoptively transferred into naïve C3H mice, which were then given B6 (donor type) or BALB/c (third party) cardiac allografts. The graft survival after adoptive transfer of splenocytes is shown (n = 7 mice for Naïve AT control group, n = 3 mice for 60 days-AT-D_type group, n = 8 mice for 100 days-AT-D_type group, n = 3 mice for 60 days-AT-Third party group, n =7 mice for 100 days-AT-Third party group, pooled from two independent experiments). Table 2 shows the detailed graft survival data. A statistical evaluation of the graft survival was performed using Kaplan–Meier curves with a comparison using log-rank tests. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.