Table 3.
Statistically significant quantitative and qualitative changes in the immune response in spleen of recipients after FR70 treatment on POD7, 60 and 100 in comparison to POD7 of the control recipients.
| Compartment | POD7 | POD60 | POD100 |
|---|---|---|---|
| Spleen | |||
| T cell-related | ↓ CTL number | ↓ CTL number | ↓ CTL number |
| CTLs | ↓ perforin mRNA | ↓ perforin mRNA | ↓ perforin mRNA |
| ↑ Treg number | ↑ Treg number | ||
| Tregs | ↑ IL-10 mRNA | ||
| DC-related | ↑ DC number | Not available | |
| TolDCs | ↑ HO-1 mRNA | ||
| ↓ iNOS mRNA | |||
| ↓CD40 mRNA/protein | ↓ CD40 mRNA | ||
| ↓MHCII mRNA/protein | |||
| ↓ CD80 mRNA/protein | ↓ CD80 mRNA | ↓ CD80 mRNA | |
| ↓ CD86 mRNA | ↓ CD86 mRNA | ↓ CD86 protein | |
| ↑ PD-L1 protein | Not available | ↑ PD-L1 protein | |
| Cytokines | ↓ IL-1β mRNA | ||
| ↓ IL-12β mRNA | ↓ IL-12β mRNA | ↓ IL-12β mRNA | |
| ↓ IL-6 mRNA | ↓ IL-6 mRNA | ↓ IL-6 mRNA |
Cells isolated from spleen on POD60 and 100 were used in a secondary transplant experiment. Long-term tolerogenic effects observed in the primary recipients were caused by time-dependent quantitative and qualitative changes in regulatory cells, which also translated in the tolerogenic memory effect and acceptance of grafts in the secondary transplant recipients; ↓ decreased levels, ↑ increased levels.