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Effect of curcumin and its analogues on p65 localization in MFC-7/HER2-5 cells. Cells were grown on coverslips and treated with compounds as indicated with the concentration of ½ IC50 and then subjected to immunofluorescent staining with p65 antibody and DAPI as described in the materials and methods. Visualizations were performed under a fluorescence microscope with 400× magnification.
