Table 3. Quantitative calculations in Hematoxylin-Eosin (HE) and Victoria Blue staining to count the number of the islets of Langerhans, the area of the islets of Langerhans, and the percentage of pancreatic beta cells (n = 3) .
Code | Treatment | The number of islets of Langerhans | The area of islets of Langerhans (µm 2 ) | The percentage of pancreatic beta cells (%) |
A | Normal rats | 9.00 ± 1.41* | 868,761 ± 135.20* | 69.83 ± 1.79* |
B | Normal rats receiving AND suspension | 7.50 ± 1.29* | 623,046 ± 13.99* | 66.12 ± 8.6* |
C | Diabetic rats receiving ASNE | 5.75 ± 2.36* | 387,161 ± 64.00* | 61.70 ± 1.12* |
D | Diabetic rats receiving AND suspension | 5.25 ± 1.50* | 681,929 ± 157.41* | 65.49 ± 0.48* |
E | Diabetic rats as the negative control | 2.75 ± 1.26 | 274,307 ± 29.7 | 40.52 ± 4.56* |
* Significantly different from the negative control (P < 0.05)
Abbreviations: AND, andrographolide; ASNE, andrographolide in self-nanoemulsifying.