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Primer sequences
| Genes | Primer sequence (5’→3′) | Product size (bp) |
|---|---|---|
| GAPDH | F:GATGGTGAAGGTCGGAGTGAAC | 100 |
| R:GTCATTGATGGCGACGATGT | 100 | |
| MyHC I | F:ATCGCTGAATCCCAGGTCAA | 92 |
| R:ACCAAGATGTGGCACGGCTA | 92 | |
| MyHC IIa | F:CACCCTGGAGCAGACAGAGA | 148 |
| R:TCCCTGGATTTGCGTGATG | 148 | |
| MyHC IIx | F:TTTCCAGACCGTGTCTGCTC | 96 |
| R:GGGATGATGCAGCGTACAAAG | 96 | |
| MyHC IIb | F:AGGGCATCGCTGGAACAGAC | 84 |
| R:CAGAAGCTGCACACGCTCAC | 84 |
GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MyHC, myosin heavy chain.
