Table 1.
Genes | Primer sequence (5′ → 3′) | Product size (bp) | Gene bank number |
---|---|---|---|
Nrf2 | F: CAGGCCGTCTTGAAGCTCATCTC | 179 | NM_205117.1 |
R: CTTGCCTCTCCTGCGTATATCTCG | |||
HMOX1 | F: ACGTCGTTGGCAAGAAGCATCC | 181 | NM_205344.1 |
R: TTGAACTTGGTGGCGTTGGAGAC | |||
NQO1 | F: TCGCCGAGCAGAAGAAGATTGAAG | 191 | NM_001277621.1 |
R: GGTGGTGAGTGACAGCATGGC | |||
SOD | F: GGTGACCTCGGCAATGTGACTG | 93 | NM_205064.1 |
R: AATGATGCAGTGTGGTCCGGTAAG | |||
GPX | F: AAGTGCTGCTGGTGGTCAACG | 155 | NM_001277853.2 |
R: GTTGGTGGCGTTCTCCTGGTG | |||
GCLC | F: GAAGCACGGCATCCTCCAGTTC | 151 | XM_419910.5 |
R: TTCTTCGCCACTCAGCACTAAGC | |||
GCLM | F: GCCATAGGCACCTCTGACCTTG | 111 | NM_001007953.1 |
R: CGGCATCACGCAACATGAAGC | |||
GAPDH | F: GGTAGTGAAGGCTGCTGCTGATG | 200 | NM_204305.1 |
R: AGTCCACAACACGGTTGCTGTATC |
Nrf2, nuclear factor erythroid 2-related factor 2; HMOX1, heme oxygennase 1; NQO1, NAD(P)H quinone dehydrogenase 1; SOD, superoxide dismutase; GPX, glutathione peroxidase; GCLC, glutamate cysteine ligase catalytic subunit; GCLM, glutamate cysteine ligase modifier subunit; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.