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. 2020 Apr 13;34(4):652–661. doi: 10.5713/ajas.19.0953

Table 1.

Primer sequences used for real-time polymerase chain reaction

Genes Primer sequence (5′ → 3′) Product size (bp) Gene bank number
Nrf2 F: CAGGCCGTCTTGAAGCTCATCTC 179 NM_205117.1
R: CTTGCCTCTCCTGCGTATATCTCG
HMOX1 F: ACGTCGTTGGCAAGAAGCATCC 181 NM_205344.1
R: TTGAACTTGGTGGCGTTGGAGAC
NQO1 F: TCGCCGAGCAGAAGAAGATTGAAG 191 NM_001277621.1
R: GGTGGTGAGTGACAGCATGGC
SOD F: GGTGACCTCGGCAATGTGACTG 93 NM_205064.1
R: AATGATGCAGTGTGGTCCGGTAAG
GPX F: AAGTGCTGCTGGTGGTCAACG 155 NM_001277853.2
R: GTTGGTGGCGTTCTCCTGGTG
GCLC F: GAAGCACGGCATCCTCCAGTTC 151 XM_419910.5
R: TTCTTCGCCACTCAGCACTAAGC
GCLM F: GCCATAGGCACCTCTGACCTTG 111 NM_001007953.1
R: CGGCATCACGCAACATGAAGC
GAPDH F: GGTAGTGAAGGCTGCTGCTGATG 200 NM_204305.1
R: AGTCCACAACACGGTTGCTGTATC

Nrf2, nuclear factor erythroid 2-related factor 2; HMOX1, heme oxygennase 1; NQO1, NAD(P)H quinone dehydrogenase 1; SOD, superoxide dismutase; GPX, glutathione peroxidase; GCLC, glutamate cysteine ligase catalytic subunit; GCLM, glutamate cysteine ligase modifier subunit; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.