Table 7.

Analytical methods for the determination of the main components of the Psilocybe genus.

Compounds	Sample (Amount)	Sample Preparation	Analytical Technique	Limits of Detection	Limits of Quantitation	Recovery (%)	Reference
Psilocin glucuronide and Psilocin	Urine (0.1mL)	Enzymatic hydrolyses (β-glucuronidase), alkaline hydrolyses (potassium hydroxide) acid hydrolysis (concentratedhydrochloric acid) and deproteinization (methanol)	LC-MS (ESI) and LC-MS/MS (ESI)	0.5 μg/L	-	-	[138]
Psilocin glucuronide	Serum (0.1mL)	Enzymatic hydrolysis (β-glucuronidase) and deproteinization (methanol)	LC-MS (ESI) and LC-MS/MS (ESI)	0.5 μg/L	-	-	[139]
Mescaline, DMT, Psilocin, Psilocybin and Salvinorin A	Hair (25 mg)	Hydrolysis (M3reagent)	UHPLC-MS/MS (ESI)	0.01–0.02 μg/g	0.03–0.05 μg/g	79.6–97.4	[67]

Caption: ESI (electrospray ionization); LC (liquid chromatography); MS (mass spectrometry); MS/MS (tandem mass spectrometry); UHPLC (ultrahigh-performance liquid chromatography).