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. 2021 Mar 5;13(5):1111. doi: 10.3390/cancers13051111

Figure 3.

Figure 3

Focal adhesion kinase (FAK) inhibition reduces glucose uptake and lactate acid production. LN229 and U251MG cells were plated on osteopontin and (A,B) FAK activation is decreased in response to siRNA mediated knockdown of αv or β3 when compared to that in cells treated with a control/scrambled siRNA (scr), or in cells treated αvβ3 blocking antibody when compared to cells treated with control isotype antibody (ctrl). (C,D) cells were then treated without or with PF562271 (PF56) or PF573228 (PF57), two FAK specific inhibitors at the indicated doses. Uptake of glucose analog 2-NBDG was reduced in cells treated with PF56 or PF57. (E,F) Lactate acid production in the culture medium was decreased in cells treated with FAK inhibitors PF56 or PF57. All experiments were repeated at least three times. Data are represented as mean ± SE, ** p < 0.01.