Figure 2.

Bacterial viability (score 1–5) of biofilms formed on dentin specimens after rinsing with different rinsing solutions. Subjects carried intraoral splints with specimens for 48 h and rinsed with different experimental solutions. In rinsing protocol 1, rinsing was performed after 3 min, 12 h, 24 h, 36 h and 47.5 h and in rinsing protocol 2 after 3 min, 12 h, 24 h and 36 h. The biofilm formed on specimens was stained with LIVE/DEAD^® Baclight™ and evaluated using a scoring system. The height of the bars corresponds to mean values and the line applied to standard deviations. Friedman test followed by Dunn’s multiple comparison test: significant differences (p < 0.05) to water are marked with a for rinsing protocol 1 and with b for rinsing protocol 2. TA = tannic acid, CHX = chlorhexidine.