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. 2021 Mar 5;13(5):1126. doi: 10.3390/cancers13051126

Figure 1.

Figure 1

Mesenchymal stem cells (MSCs) from dogs and mice transform spontaneously after long-term culture. (A) MSCs isolated from NMRI or C57BL/6J mice undergo spontaneous transformation after long-term in vitro culture based on cumulative population doubling (Cum. PD). Example of transformation event in B6_10 is indicated by an arrow. (B) Representative images of murine and canine MSCs. Late passage murine MSCs show a higher nuclear to cytoplasm ratio, as evident from the nuclear enlargement, compared to early passage MSCs. Scalebar represents 100 µm. Karyotyping showed late passage MSCs have an increased number of chromosomes per cell after DAPI staining, as murine and canine cells normally have 40 or 78 chromosomes, respectively. (C) MSCs isolated from a 7-year-old Rottweiler transform spontaneously after long-term ex vivo culture (OSBMSC1), indicated with an arrow, at each data point, cells were trypsinized and passaged. (D) DNA content analysis by flow cytometry shows that late passage canine MSCs OSBMSC1 have become aneuploid.