Table 2.
Flow Cytometric Analysis of Annexin V/PI Staining Performed on HeLa Cells after 24, 48 and 72 h of L1 and TGF-β Treatment.
| Live | Early Apoptotic | Late Apoptotic | Death | |
|---|---|---|---|---|
| CTRL 24 h | 92.95 ± 0.55 | 2.05 ± 0.41 | 1.55 ± 0.05 | 3.46 ± 0.94 |
| TGF-β | 94.00 ± 1.10 | 1.70 ± 0.70 | 1.70 ± 0.26 | 2.87 ± 0.16 |
| TGF-β + L1 | 88.40 ± 3.40 | 4.04 ± 2.62 | 3.90 ± 0.61 | 3.66 ± 0,18 |
| L1 | 85.00 ± 0.40 *+ | 6.02 ± 0.60 | 4.94 ± 0.73 | 4.03 ± 0.26 |
| CTRL 48 h | 93.30 ± 0.50 | 2.44 ± 0.02 | 3.04 ± 0.56 | 1.25 ± 0.10 |
| TGF-β | 95.05 ± 0.35 | 2.28 ± 0.20 | 1.61 ± 0.49 | 1.03 ± 0.07 |
| TGF-β + L1 | 83.20 ± 1.60 *+ | 11.53 ± 2.73 *+ | 3.52 ± 0.62 | 1.76 ± 0.13 |
| L1 | 81.70 ± 0.70 *+ | 11.89 ± 0.72 *+ | 4.42 ± 1.15 | 1.95 ± 0.27 |
| CTRL 72 h | 93.20 ± 0.70 | 2.19 ± 0.18 | 3.09 ± 0.67 | 1.52 ± 0.26 |
| TGF-β | 92.95 ± 1.65 | 3.24 ± 1.51 | 2.36 ± 0.13 | 1.48 ± 0.26 |
| TGF-β + L1 | 76.65 ± 1.35 **++ | 15.91 ± 0.41 *+ | 6.64 ± 0.41 | 4.99 ± 0.53 |
| L1 | 74.35 ± 1.45 **++ | 17.34 ± 1.54 **++ | 2.43 ± 0.11 | 1.66 ± 0.20 |
The data are presented from three independent experiments after 24 h, 48 h, and 72 h of L1 (10 µM) and TGF-β treatment as mean percentage ± SD. Significantly different * p < 0.05, ** p < 0.01 vs. untreated cells (control); + p < 0.05, ++ p < 0.01 vs. TGF-β.