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. 2021 Mar 4;22(5):2562. doi: 10.3390/ijms22052562

Figure 1.

Figure 1

Limit of detection (LOD) and reproducibility of digital PCR (dPCR) for quantitative assessment of the KIT D816V variant allele fraction (VAF). (A) dPCR of a dilution series of a patient sample with 50% VAF. Single points represent merged measurements from multiple chips (n = 3). Dilution results are linear down to 0.1% VAF. (B) Reproducibility of four patient samples from 0.1 to 7.6% KIT D816V VAF (measured with at least 3 replicates) showing a coefficient of variation (CV) below 20% for all samples.