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. 2021 Mar 5;22(5):2647. doi: 10.3390/ijms22052647

Figure 2.

Figure 2

(A) The nuclear export sequences (NES) of Zyxin family proteins. To investigate the conservation of NES in proteins from other organisms, we aligned the human protein sequence using BLAST against the non-redundant protein sequence database. The resultant sequences obtained from BLAST were subjected to multiple sequence alignment, presented using WEBLOGO (B) The NES sequence for each protein was identified through the NetNES webserver, and the sequences were aligned using MultAlin. (C) WEBLOGO was used to investigate the conservation of residues in the sequences, which suggested the presence of signature LxxLxL/LxxxLxL, (L = L/V/I/F/M; x = any amino acid) repeat, in NES sequence across all the members of Zyxin family except zyxin and LPP which showed LxxxLxxL/LxxLxxL consensus sequence. (D) The nuclear localization sequence (NLS) for the Zyxin family members was fetched by the NLStradamus webserver with a prediction cut-off set at a recommended value of 0.6. The conservation of predicted sequences was analysed using MulitAlin.