Table 1.

Patient overview. Patient-derived colorectal cancer spheroids (P1–P12), organoids (O1–O3), xenografts (X1, X2), and primary colorectal cancer samples (T1–T3) used for single-cell RNA-sequencing. X indicates mutation, - indicates wild type. f, female; m, male; met, metastasis; MS, microsatellite; MSI, microsatellite instable; MSS, microsatellite stable; N/A, not available.

Patient	Sex	Origin	Site	Stage (UICC)	MS status	TP53	APC	KRAS
P1	m	liver met	Rectum	IV	MSS	X	X	X
P2	m	lung met	Caecum	IV	MSS	X	X	X
P3	f	liver met	Rectum	IV	MSS	X	X	X
P4	f	liver met	Ascending colon	IV	MSI	X	X	X
P5	f	primary	Transverse colon	IV	MSS	-	-	-
P6	f	primary	Caecum	IV	MSS	X	-	-
P7	m	liver met	Sigmoid	IV	MSS	-	X	X
P8	m	liver met	Caecum	IV	MSS	X	-	-
P9	m	primary	Rectum	IV	MSS	X	-	X
P10	m	primary	Sigmoid	IIIB	MSS	N/A	N/A	N/A
P11	m	primary	Rectum and caecum	IIIB	MSS	-	-	-
P12	m	primary	Rectum and transverse colon	II	MSI	X	X	X
O1	f	liver met	Sigmoid	IV	MSS	X	X	X
O2	f	liver met	Caecum	IV	MSS	X	X	X
O3	f	liver met	Ascending colon	IV	MSI	-	X	-
X1	m	primary	Rectum	I	N/A	N/A	N/A	N/A
X2	m	primary	Ascending colon	II	MSI	N/A	N/A	-
T1	m	primary	Sigmoid	III	N/A	N/A	N/A	N/A
T2	m	primary	Ascending colon	IV	N/A	N/A	N/A	N/A
T3	m	primary	Ascending colon	IVa	MSS	N/A	N/A	X