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Preliminary experiments for subsequent quartz crystal microbalance with dissipation (QCM-D) analyses. (A) Biotin-labelling of agalsidase-α, agalsidase-β and Moss-AGAL. 10 ng respective enzymes (biotin-labeled and unlabeled) were used for western blot analysis. (B) Schematic workflow for crystal labelling and measurements. (C) QCM-D monitoring to control and measure the binding affinity of a commercial rabbit-derived antibody against agalsidase-α, agalsidase-β, and Moss-AGAL.
