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. 2021 Mar 4;22(5):2579. doi: 10.3390/ijms22052579

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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In vivo neuroprotective activities of ahMNCs. (A) Immunohistochemistry against NeuN. Red arrows indicate NeuN positive neurons in close proximity to the damaged lesions both rostrally and caudally and the distance between these neurons was evaluated as part of this experiment. Scale bar = 400 µm and 40 µm in the lower and higher magnifications, respectively. (B) These distances were quantified and then compared. (C) Correlation analysis revealed a significant negative relationship between the distance between the NeuN positive cells and the BBB score. (D) Tuj1 immunoreactivity was evaluated in five areas: Dorsal, Epicenter, Rostral, Ventral, and Caudal. (E) Imaging revealed which of these areas were experiencing this immunoreactivity. (F) The relative intensity of these images was compared to determine the extent of Tuj1 immunoreactivity in each area. (G) Correlation analysis revealed a significant positive relationship between the relative area of Tuj1 reactivity and the BBB score. (H,I) These immunoreactive areas were independently quantified for the Dorsal and Epicenter lesions (H) and Rostral, Ventral, and Caudal lesions (I) and then compared. (J) Correlation analysis revealed a significant positive relationship between the Tuj1-positive area in the Rostral, Ventral, and Caudal lesions and the BBB score. Height = Average, Error bar = Standard Error. *, P < 0.05.