Figure 2.

Profiling non-coding RNAs with transcriptome sequencing in B-ALL. In the study of B-ALL, input sample types will vary and can include biological specimens such as the bone marrow, peripheral blood, cord blood, or plasma. RNA is harvested from cells and then assessed for quality. Extracted total RNA from one sample can then be split and used for downstream library construction to profile both small- and long- ncRNAs. Small RNA-sequencing library protocols incorporate additional steps that are required to sequence small RNA species, typically <30 nucleotides. Long non-coding RNAs, as well as mRNAs, are detected with the conventional RNA-Sequencing library preparation protocol. Created with BioRender.com.