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. 2021 Mar 5;22(5):2607. doi: 10.3390/ijms22052607

Figure 2.

Figure 2

Design and efficiency testing of gRNAs. (A) Sequence of exon 2 of NR2E3 in the DNA of the NR2E3-adRP patient showing the position of the c.166G>A mutational site in red. The position of gRNA1 is indicated by a green line and the corresponding NGG PAM sequence is in bold and underlined in green. The position of gRNA2 is indicated by a blue line and the corresponding NGA PAM sequence is indicated in bold and underlined in blue. (B) Gel electrophoresis of the T7E1 assay in G56R and WT iPSCs that were mock-transfected or transfected with gRNA1 or gRNA2. The minus sign indicates the absence, and the plus sign indicates the presence, of T7E1. Molecular weight (MW) marker, 100 bp ladder.