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. 2021 Mar 5;22(5):2645. doi: 10.3390/ijms22052645

Figure 6.

Figure 6

Nckx3 regulates NF-κB activation by targeting p53. (A) NF-κB p65 and p53 and GAPDH protein expression levels in the colon of WT and Nckx3 KO mice were detected by Western blotting. (B,C) Quantification of (A). The protein level of p65 was increased in the WT + DSS compared to the WT group, but not significantly. However, the Nckx3 KO +DSS groups showed a markedly higher protein level of p65 than in the WT, WT + DSS and Nckx3 KO groups. In addition, the WT, WT + DSS and Nckx3 KO groups showed higher protein levels of p53 than the WT group (n = 10 per group). (D) RT-qPCR analysis NF-κB p65 and p53 mRNA expression levels in colon of WT and Nckx3 KO mice (n = 10 per genotype). Data represent the mean ± SEM. Statistical significance was determined by one-way ANOVA with Bonferroni correction. * p < 0.05 vs. WT, ** p < 0.01 vs. WT, *** p < 0.001 vs. WT, # p < 0.05 Nckx3 KO + DSS vs. Nckx3 KO, ## p < 0.01 Nckx3 KO + DSS vs. Nckx3 KO, ### p < 0.01 Nckx3 KO + DSS vs. Nckx3 KO, & p < 0.05 WT + DSS vs. Nckx3 KO + DSS, && p < 0.01 WT + DSS vs. Nckx3 KO + DSS.